stat2 defective mutant daudi cells  (Absolute Biotech Inc)

Journal: Autophagy

Article Title: Type I interferons induce autophagy in certain human cancer cell lines

doi: 10.4161/auto.23921

Figure Lengend Snippet: Figure 2. IFNA2c induced autophagy in Daudi cells, but not in STAT2-deficient Daudi. ( A ) Direct effect of IFNA2c on induction of autophagy. Daudi cells were cultured for 24 h in supernatants from either mock-treated Daudi cells or cells treated with 0.36 ng/mL of IFNA2c for 24 h in the presence or absence of anti-IFNAR2-mAb A10. Lanes: (1) molecular weight marker; (2) negative control, cells plus supernatant from mock-treated cells; (3) negative control, cells plus supernatant from mock-treated cells and anti-IFNAR2 – mAb A10; (4) cells plus supernatant from IFNA2c-treated cells; (5) cells plus supernatant from IFNA2c-treated cells and anti-IFNAR2 – mAb. Data are representative of two individual experiments. Ratios of MAP1LC3 were calculated as the division of the ratio of induced MAP1LC3-I to induced MAP1LC3-II by the ratio of basal MAP1LC3-I to basal MAP1LC3-II, and the numbers are shown below MAP1LC3 lanes. ( B ) Detection of MAP1LC3-I and MAP1LC3-II, SQSTM1, p-STAT1, p-STAT2, p-RPS6 after 48 h treatment of STAT2-defective mutant Daudi cells with IFNA2c. STAT2-defective mutant Daudi cells were incubated with or without IFNA2c for 48 h. Lanes: (1) molecular weight marker; (2) negative control, untreated cells; (3) IFNA2c (3.6 ng/mL). Data are representative of two individual experiments. Ratios of MAP1LC3 were calculated as the division of the ratio of induced MAP1LC3-I to induced MAP1LC3-II by the ratio of basal MAP1LC3-I to basal MAP1LC3-II, and the numbers are shown below the MAP1LC3 lanes.

Article Snippet: STAT2-defective mutant Daudi cells were purchased from KeraFAST (EH0001).

Techniques: Cell Culture, Molecular Weight, Marker, Negative Control, Mutagenesis, Incubation